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BACKGROUND: It has been shown that Qiang Chang Heji (QCHJ) hadsome protective effects in rats with experimental brain injuries and couldimprove some hemorheological indices.OBJECTIVE: To observe effects of QCHJ on peripheral platelet in rats with experimental right brain insufficiency of blood supply.DESIGN: A randomized and controlled experiment.SETTING: Central Laboratory, General Hospital of Chengdu Military Area Command of Chinese PLA.MATERIALS: Totally 60 SD white rats (either sex, werghing from 150 g to 170 g) were used. They were divided randomly into 3 groups: sham-operation group (n=8), control group (n=18) and treatment group (n=34).QCHJ:Chinese herbal mixture, equal dosage of both Rhizoma seu Radix Notopterygii and Rhizoma Acori Graminei were decocted twice, each for 30minutes with mild fire after boiling. The two decoctions were mixed and concentrated to 1g raw herb per milliliter.METHODS: The experiment was carried out in Central Laboratory, General Hospital of Chengdu Military Area Command of Chinese PLA from July to September 2004. The right carotid artery (CA) of rats were separated,ligaturewas done in control and treatment groups ;but no ligature in sham operation group. Medication (po. by gastric perfusion) was given since the day of operation. In treatment group, 10 rats took QCHJ 10 g/kg per day for3 days; and others took 3.3, 6.7, 10 g/kg per day for 7 days respectively, (8 rats for each dose). In control group, equal volume physiological saline was given daily for 3 or 7 days. In sham operation group, equal volume physiological saline was given daily for 3 days. On the 3rd and 7th days after operation, platelet count (Plt), mean platelet volume (MPV), platelet distribution width (PDW) and platelet-large cell ratio (P-LCR) of platelet were determined with a Bakeman Blood Cell Auto-analyzer. t test was used to compare the difference.MAIN OUTCOME MEASURES:Effects of QCHJ on peripheral platelet in rats with brain insufficiency of blood in brain.RESULTS: ①Day 3 after operation: PLt were similar between control group and sham-operation group (P ＞ 0.05); MPV, PDW and P-LCR were higher in control group than that in sham operation group (P ＜ 0.01); in QCHJ treated group (10 g/kg per day), MPV, PDW, and P-LCR were significantly lower than that in control group (P ＜ 0.01), and they were similar to that in sham operation group. ② Day 7 after operation: MPV, PDW, and P-LCR in control group were similar to that in sham operation group. In addition, P-LCR in control and QCHJ treatment group (3.3, 6.7, 10.0 g/kg per day) were lower than that in control group on the 3rd day of operation (P ＜ 0.01).CONCLUSION: Experimental brain injury induced by block blood supply may result in peripheral platelet abnormities. QCHJ has been shown to improve some of the abnormities.
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BACKGROUND: It has been discovered in experimental researches that qiang chang heji acts on prevention and treatment of brain injuries induced by insufficient blood supply and improvement of blood rhelogical property.OBJECTIVE: To study on the effects of qiang chang heji on peripheral red blood cell in rats with insufficient blood supply to brain so as to probe into its mechauism on improving blood rhelogical property.DESIGN: A randomized controlled trial.SETTING: Central Laboratory of General Hospital of Chengdu Military Area Command of Chinese PLA.MATERIALS: The experiment was performed in Central Laboratory of General Hospital of Chengdu Military Area Command of Chinese PLA from July to September 2003. Totally 50 SD white rats of either sex were employed, weighted varied from 150 g to 170 g.METHODS: The common carotid artery(CA) on the right side was ligatured to induce insufficient blood supply in the brain. The control and qiang chang heji(QCHJ) group were designed for comparison. On the same day of CA ligature, in treatment group, QCHJ of various dosages were applied daily for gastric perfusion; and in the control, physiological saline was applied. Three or seven days later, the parameters of peripheral red blood cell and its rhelogical property were analyzed with equipment.the sham-operation group, mean cell hemoglobin (MCH), haematocrit(HCT) (%) and RBC aggregation index were in the tendency of increase and mean cell hemoglobin concentration (MCHC) (g/L) was increased obviously (375±7 vs 363±13) (P ＜ 0.05) .In the treatment group of 10 g/kg QCHJ, HGB(139.2±12.4 vs 153.6±9.6), MCHC(355±7 vs 375±7) and haematocrit(HCT) (35.4 ±0.9 vs 42.8 ±4.5) were significantly lower the control compared with the sham-operation group, both rigidity index and aggregation index of RBC were in the tendency of further increase. In treatment of QCHJ 10 g/kg, MCHC(354±4 vs 361 ±4), RBC rigidity index (1.02±0.35 vs 4.54±3.04) and aggregation index (2.76±0.66 vs 4.67 ± 1.21 ) were significantly lower than the control( P ＜ 0.05).CONCLUSION: Treatment with QCHJ can prevent changes in peripheral red blood cell in rats with insufficient blood supply in the brain and reduce MCHC and RBC rigidity index and aggregation index so as to improve RBC deformability and rhelogical property.
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<p><b>OBJECTIVE</b>To show the changes of heat shock protein 70(HSP70) expression at cellular level in different heat exposure stages and the significance of HSP70 expression in heat exposure organism.</p><p><b>METHOD</b>The heat exposure model was established in rats with artificial hot climatic chamber[34 +/- 1) degree C, RH 60%]. SD rats were randomly divided into control groups(C) and heat exposure groups(A), and into subgroups including 2, 7, 14, 28 d stages from each one of the groups. The immuno-histochemistry was used to detect HSP70 expression in rat liver and cardiac muscle, and photography analytic software was used to analyze HSP70 expression in liver and cardiac cells.</p><p><b>RESULTS</b>The expression intensity of HSP70 in heat exposure groups(gray values of liver were 137.0 +/- 5.1, 137.0 +/- 5.2, 137.8 +/- 7.1, 139.2 +/- 5.2 respectively; of cardiac muscle 156.1 +/- 4.4, 155.1 +/- 6.2, 155.4 +/- 4.5, 156.2 +/- 5.1 respectively) was stronger than that in control groups(P < 0.01 or P < 0.05) during all stages of the heat exposure; There was no significant difference in expression intensity of HSP70 among various stages of heat exposure; after 2 d of heat exposure, HSP70 expression in cell nuclei of the liver and cardiac muscle cells was stronger than that in cytoplasm in heat exposure group; HSP70 expression in Kupffer's cells of liver was also stronger than that of control(P < 0.05), but not on 7, 14 and 28 d; the activities of alanine aminotransferase(ALT), aspartate aminotransferase(AST), creatine kinase(CK) and hydroxybutyrate dehydrogenase(HBD) showed an increase on 2 d and 28 d of heat exposure.</p><p><b>CONCLUSION</b>The vital organs would be damaged on 2 d of heat exposure. High expression of HSP70 at this stage may be a marker of cell damage; Increased HSP70 expression on 7-14 d of heat exposure may play an important role in adaptation to heat, while long term(28 d) heat exposure, the protection of HSP70 from tissue damage may not be enough.</p>
Subject(s)
Animals , Rats , Adaptation, Physiological , HSP70 Heat-Shock Proteins , Heat Stress Disorders , Metabolism , Liver , Metabolism , Myocytes, Cardiac , Metabolism , Rats, Sprague-Dawley , Time FactorsABSTRACT
OBJECTIVE： The preventive and therapeutic effects of a compound traditional Chinese medicine preparation， Yingganfukang(YGFK)， on mice experimental liver fibrosis were studied METHODS： The acute liver damage and fibrosis rat models were established by giving single or multi－ dose of CCl4 orally and the liver dysimmunity model was established by giving BCG and LPS intravenously Rats were divided into YGFK group and control group The serum ALT， AST and ALP of the mice were examined The liver tissues taken from the liver fibrosis mice were committed to pathological examination RES_ ULTS： The serum ALT， ALP of the YGFK(2g/kg· d) group and control group were （ 6 153± 3 491） IU/L、 （ 202± 24） IU/L and （ 9 275± 2 744） IU/L、 （ 421± 67） IU/L， respectively in the acute liver damage mice with significant differences
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In this report, the immunopharmacological effects of Polyactin A ( PAA ) were presented. When the SRBC immunized mice were administered daily 2. 5~40mg/kg ( ip ) of PAA for 3 or 5 days, the serum level of hemolysin, the antibody production in spleen and the number of nucleated cells of spleen were enhanced significantly; and the efficacy-dose dependency was evident in these experiments。 In spite of these effects, PAA didn't affect the kinetics of circulating antibody level.In other experiments, it was found that the efficiency was more potent when PAA was given before or simultaneously with SRBC immunization than given after.